Pattern of chick gene activation in chick erythrocyte heterokaryons.

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Pattern of chick gene activation in chick erythrocyte heterokaryons

The reactivation of chicken erythrocyte nuclei in chick-mammalian heterokaryons resulted in the activation of chick globin gene expression. However, the level of chick globin synthesis was dependent on the mammalian parental cell type. The level of globin synthesis was high in chick erythrocyte-rat L6 myoblast heterokaryons but was 10-fold lower in chick erythrocyte-mouse A9 cell heterokaryons....

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Migration of rat RNA polymerase I into chick erythrocyte nuclei undergoing reactivation in chick-rat heterokaryons

Transcriptionally inactive chick erythrocyte nuclei were reactivated by Sendai virus-induced fusion of erythrocytes with rat L6J1 myoblasts. We used antibodies to trace the appearance of a specific protein engaged in transcription of a defined class of genes, those coding for rRNA, during reactivation. Using immunofluorescence microscopy, we found increasing amounts of rat RNA polymerase I to a...

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Migration of Rat RNA Polymerase I Nuclei Undergoing Reactivation in into Chick Chick-Rat Erythrocyte Heterokaryons

Transcriptionally inactive chick erythrocyte nuclei were reactivated by Sendal virusinduced fusion of erythrocytes with rat L6J1 myoblasts. We used antibodies to trace the appearance of a specific protein engaged in transcription of a defined class of genes, those coding for rRNA, during reactivation. Using immunofluorescence microscopy, we found increasing amounts of rat RNA polymerase I to ap...

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Chick-erythrocyte nucleus reactivation in heterokaryons: suppression by inhibitors of proteolytic enzymes.

Reactivation of chick-erythrocyte nuclei in heterokaryons (obtained by Sendai virus-induced fusion of chick erythrocytes with HeLa cells) is suppressed by specific inhibitors of trypsin and trypsin-like enzymes. N-alpha-tosyl-L-lysyl-chloromethane and N-alpha-tosyl-L-arginine methylester inhibit erythrocyte nuclear enlargement and suppress RNA and DNA synthesis in nuclei of erythrocytes and HeL...

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Appearance and origin of snRNP antigens in chick erythrocyte nuclei reactivated in heterokaryons.

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1982

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.95.3.885